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blue fluorescence  (Beyotime)


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    Structured Review

    Beyotime blue fluorescence
    Blue Fluorescence, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 32382 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+(blue+fluorescence/DAPI/pmc12131344-69-0-4
    Average 99 stars, based on 32382 article reviews
    blue fluorescence - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Staining:

    Article Title: Lipid Nanoparticular Codelivery System for Enhanced Antitumor Effects by Ferroptosis-Apoptosis Synergistic with Programmed Cell Death-Ligand 1 Downregulation.
    Article Snippet: Intrinsic or acquired resistance to chemical drugs severely limits their therapeutic efficacy in cancer treatment.. Various intracellular antioxidant molecules, particularly glutathione (GSH), play a crucial role in maintaining intracellular redox homeostasis by mitigating the overproduced reactive oxygen species (ROS) due to rapid cell proliferation.. Notably, these antioxidants also eliminate chemical-druginduced ROS, eventually diminishing their cytotoxicity and rendering them less effective.

    Article Title: Chinese leek-derived extracellular vesicles ameliorate sarcopenia by regulating mitochondrial biogenesis and autophagy via AMPK and maintaining myosin homeostasis
    Article Snippet: .. Then, C2C12 cells were incubated with working solution at 37 °C for 5 min followed by 4 °C for 15 min. Cytoskeleton was stained with FITC-phalloidin (green fluorescence, Yesen, China), nuclei were counterstained with DAPI (blue fluorescence, Beyotime, China). ..

    Article Title: 3D-printed NIR-II-responsive CaCuSi4O10/PCL/HA elastic scaffold for integrated photothermal therapy, antibacterial activity, and bone regeneration
    Article Snippet: To address the dual challenges of post-surgical bone tumor recurrence and extensive bone defects, this study developed a multifunctional photothermal bone scaffold integrating tumor elimination and osteogenesis promotion.. CaCuSi4O10 nanosheets (EB NSs), exhibiting rapid NIR-II-responsive photothermal conversion, were synthesized and incorporated into a high-ceramic-content (70 wt% hydroxyapatite) elastic scaffold via pneumatic extrusion-based 3D printing.. The scaffold demonstrated excellent mechanical adaptability, enabling shape customization for irregular bone defects.

    Article Title: Embryonic lethal abnormal vision like 1-stabilized histone deacetylase 6 promotes hepatic stellate cell activation to accelerate liver fibrosis progression through ribosomal protein S5 downregulation
    Article Snippet: LX2 cells were seeded in 96-well plates and cultured with CCK8 reagent (C0041, Beyotime) after 48 h. Cell viability was assessed using a microplate reader (SpectraMax i3x, Molecular Devices, LLC, Sunnyvale, CA, USA) at 450 nm. .. LX2 cells were stained with EdU solution (red fluorescence) and 4’,6-diamidino-2-phenylindole (DAPI) (blue fluorescence; C1002, Beyotime) using EdU Cell Proliferation Kit (C-10310-1, RiboBio, Guangzhou, China). .. Cell images were observed, and EdU-positive cells were analyzed under a fluorescence microscope (BX53, Olympus, Tokyo, Japan) with ImageJ software (version 1.8.0, NIH).

    TUNEL Assay:

    Article Title: Lipid Nanoparticular Codelivery System for Enhanced Antitumor Effects by Ferroptosis-Apoptosis Synergistic with Programmed Cell Death-Ligand 1 Downregulation.
    Article Snippet: Intrinsic or acquired resistance to chemical drugs severely limits their therapeutic efficacy in cancer treatment.. Various intracellular antioxidant molecules, particularly glutathione (GSH), play a crucial role in maintaining intracellular redox homeostasis by mitigating the overproduced reactive oxygen species (ROS) due to rapid cell proliferation.. Notably, these antioxidants also eliminate chemical-druginduced ROS, eventually diminishing their cytotoxicity and rendering them less effective.

    Fluorescence:

    Article Title: Lipid Nanoparticular Codelivery System for Enhanced Antitumor Effects by Ferroptosis-Apoptosis Synergistic with Programmed Cell Death-Ligand 1 Downregulation.
    Article Snippet: Intrinsic or acquired resistance to chemical drugs severely limits their therapeutic efficacy in cancer treatment.. Various intracellular antioxidant molecules, particularly glutathione (GSH), play a crucial role in maintaining intracellular redox homeostasis by mitigating the overproduced reactive oxygen species (ROS) due to rapid cell proliferation.. Notably, these antioxidants also eliminate chemical-druginduced ROS, eventually diminishing their cytotoxicity and rendering them less effective.

    Article Title: Chinese leek-derived extracellular vesicles ameliorate sarcopenia by regulating mitochondrial biogenesis and autophagy via AMPK and maintaining myosin homeostasis
    Article Snippet: .. Then, C2C12 cells were incubated with working solution at 37 °C for 5 min followed by 4 °C for 15 min. Cytoskeleton was stained with FITC-phalloidin (green fluorescence, Yesen, China), nuclei were counterstained with DAPI (blue fluorescence, Beyotime, China). ..

    Article Title: Modified human skin cell isolation protocol and its influence on keratinocyte and melanocyte culture
    Article Snippet: Melanocytes were then incubated with recombinant anti-microphthalmia-associated transcription factor (MITF) (ab3201, Abcam, United Kingdom) and anti-tyrosinase-related protein-1 (TRP1) (ab178676, Abcam, United Kingdom) antibodies at a 1:200 dilution overnight at 4 °C, followed by further incubation at room temperature for 2 h with a goat secondary antibody to mouse IgG (Alexa Fluor 594) (A-11005, Thermo Fisher Scientific, USA) and a donkey secondary antibody to rabbit IgG at a 1:200 dilution. .. Nuclear DNA was labeled blue with 4′,6-diamidino-2-phenylindole (DAPI) (Beyotime, Shanghai, China) under a fluorescence microscope. ..

    Article Title: 3D-printed NIR-II-responsive CaCuSi4O10/PCL/HA elastic scaffold for integrated photothermal therapy, antibacterial activity, and bone regeneration
    Article Snippet: To address the dual challenges of post-surgical bone tumor recurrence and extensive bone defects, this study developed a multifunctional photothermal bone scaffold integrating tumor elimination and osteogenesis promotion.. CaCuSi4O10 nanosheets (EB NSs), exhibiting rapid NIR-II-responsive photothermal conversion, were synthesized and incorporated into a high-ceramic-content (70 wt% hydroxyapatite) elastic scaffold via pneumatic extrusion-based 3D printing.. The scaffold demonstrated excellent mechanical adaptability, enabling shape customization for irregular bone defects.

    Article Title: Embryonic lethal abnormal vision like 1-stabilized histone deacetylase 6 promotes hepatic stellate cell activation to accelerate liver fibrosis progression through ribosomal protein S5 downregulation
    Article Snippet: LX2 cells were seeded in 96-well plates and cultured with CCK8 reagent (C0041, Beyotime) after 48 h. Cell viability was assessed using a microplate reader (SpectraMax i3x, Molecular Devices, LLC, Sunnyvale, CA, USA) at 450 nm. .. LX2 cells were stained with EdU solution (red fluorescence) and 4’,6-diamidino-2-phenylindole (DAPI) (blue fluorescence; C1002, Beyotime) using EdU Cell Proliferation Kit (C-10310-1, RiboBio, Guangzhou, China). .. Cell images were observed, and EdU-positive cells were analyzed under a fluorescence microscope (BX53, Olympus, Tokyo, Japan) with ImageJ software (version 1.8.0, NIH).

    Article Title: A time-programmed therapy for enhanced cartilage regeneration in immunocompetent animals
    Article Snippet: After washing with PBS, cells were incubated with 266 species-specific secondary antibodies: Alexa Fluor 594-conjugated anti-rabbit IgG 267 (1:500, Invitrogen) and Alexa Fluor 488-conjugated anti-mouse IgG (1:500, 268 Invitrogen) for 1 h at room temperature in the dark. .. Nuclei were counterstained with 269 DAPI (blue, Beyotime, China) for 5 min. Coverslips were mounted with antifade 270 reagent and imaged using a confocal fluorescence microscope (Leica Microsystems, 271 Germany). ..

    Article Title: circ_0002456/FUS interaction inhibits NF-κB signaling to attenuate DNA damage and inflammatory responses in hDPSCs
    Article Snippet: .. Blue fluorescence (DAPI, C1005, Beyotime, China) and red fluorescence (circ_0002456, Cy3, RiboBio, China) were used to indicate the cell nuclei and circ_0002456, respectively. ..

    Article Title: Primary cilia integrity governs TRPV4-mediated NF-κB/COL2 signaling in osteoarthritis pathogenesis.
    Article Snippet: .. DAPI-containing mounting medium (P0131, Beyotime, blue fluorescence) was used to mount the slides. .. Images were captured using a ZEISS LSM 900 confocal microscope, and fluorescence intensity and cilium length were quantified using Fiji software.

    Incubation:

    Article Title: Chinese leek-derived extracellular vesicles ameliorate sarcopenia by regulating mitochondrial biogenesis and autophagy via AMPK and maintaining myosin homeostasis
    Article Snippet: .. Then, C2C12 cells were incubated with working solution at 37 °C for 5 min followed by 4 °C for 15 min. Cytoskeleton was stained with FITC-phalloidin (green fluorescence, Yesen, China), nuclei were counterstained with DAPI (blue fluorescence, Beyotime, China). ..

    Labeling:

    Article Title: Modified human skin cell isolation protocol and its influence on keratinocyte and melanocyte culture
    Article Snippet: Melanocytes were then incubated with recombinant anti-microphthalmia-associated transcription factor (MITF) (ab3201, Abcam, United Kingdom) and anti-tyrosinase-related protein-1 (TRP1) (ab178676, Abcam, United Kingdom) antibodies at a 1:200 dilution overnight at 4 °C, followed by further incubation at room temperature for 2 h with a goat secondary antibody to mouse IgG (Alexa Fluor 594) (A-11005, Thermo Fisher Scientific, USA) and a donkey secondary antibody to rabbit IgG at a 1:200 dilution. .. Nuclear DNA was labeled blue with 4′,6-diamidino-2-phenylindole (DAPI) (Beyotime, Shanghai, China) under a fluorescence microscope. ..

    Microscopy:

    Article Title: Modified human skin cell isolation protocol and its influence on keratinocyte and melanocyte culture
    Article Snippet: Melanocytes were then incubated with recombinant anti-microphthalmia-associated transcription factor (MITF) (ab3201, Abcam, United Kingdom) and anti-tyrosinase-related protein-1 (TRP1) (ab178676, Abcam, United Kingdom) antibodies at a 1:200 dilution overnight at 4 °C, followed by further incubation at room temperature for 2 h with a goat secondary antibody to mouse IgG (Alexa Fluor 594) (A-11005, Thermo Fisher Scientific, USA) and a donkey secondary antibody to rabbit IgG at a 1:200 dilution. .. Nuclear DNA was labeled blue with 4′,6-diamidino-2-phenylindole (DAPI) (Beyotime, Shanghai, China) under a fluorescence microscope. ..

    Article Title: 3D-printed NIR-II-responsive CaCuSi4O10/PCL/HA elastic scaffold for integrated photothermal therapy, antibacterial activity, and bone regeneration
    Article Snippet: To address the dual challenges of post-surgical bone tumor recurrence and extensive bone defects, this study developed a multifunctional photothermal bone scaffold integrating tumor elimination and osteogenesis promotion.. CaCuSi4O10 nanosheets (EB NSs), exhibiting rapid NIR-II-responsive photothermal conversion, were synthesized and incorporated into a high-ceramic-content (70 wt% hydroxyapatite) elastic scaffold via pneumatic extrusion-based 3D printing.. The scaffold demonstrated excellent mechanical adaptability, enabling shape customization for irregular bone defects.

    Article Title: A time-programmed therapy for enhanced cartilage regeneration in immunocompetent animals
    Article Snippet: After washing with PBS, cells were incubated with 266 species-specific secondary antibodies: Alexa Fluor 594-conjugated anti-rabbit IgG 267 (1:500, Invitrogen) and Alexa Fluor 488-conjugated anti-mouse IgG (1:500, 268 Invitrogen) for 1 h at room temperature in the dark. .. Nuclei were counterstained with 269 DAPI (blue, Beyotime, China) for 5 min. Coverslips were mounted with antifade 270 reagent and imaged using a confocal fluorescence microscope (Leica Microsystems, 271 Germany). ..

    Derivative Assay:

    Article Title: 3D-printed NIR-II-responsive CaCuSi4O10/PCL/HA elastic scaffold for integrated photothermal therapy, antibacterial activity, and bone regeneration
    Article Snippet: To address the dual challenges of post-surgical bone tumor recurrence and extensive bone defects, this study developed a multifunctional photothermal bone scaffold integrating tumor elimination and osteogenesis promotion.. CaCuSi4O10 nanosheets (EB NSs), exhibiting rapid NIR-II-responsive photothermal conversion, were synthesized and incorporated into a high-ceramic-content (70 wt% hydroxyapatite) elastic scaffold via pneumatic extrusion-based 3D printing.. The scaffold demonstrated excellent mechanical adaptability, enabling shape customization for irregular bone defects.



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